Effects of Ceftiofur and Chlortetracycline Treatment ...
2013-11-19 · DNA from NTS E. coli isolates was extracted for genotypic analysis by suspending a colony in 500 µl of nuclease-free water (Qiagen, Valencia, CA) and then heating the suspension at 95°C for 10 minutes. The NTS E. coli DNA samples were stored at −20°C for further qualitative detection of tet(A), tet(B), and bla CMY-2 genes.
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